Cdk6 Rabbit Polyclonal Antibody (ER40101)
Catalog# ER40101
Rabbit Polyclonal to CDK6
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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Monkey
Lane 1: HeLa cell lysate
Lane 2: SK-OV-3 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40101) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Cdk6 Rabbit Polyclonal Antibody (ER40101)
Immunogen
Synthetic peptide within human cdk6 aa 267-326 / 326.
Host
Rabbit
Positive Control
HeLa cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, K562 cell lysate, COS-1 cell lysate, Hela, HepG2, human lung cancer tissue, mouse thymus tissue, rat thymus tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:200
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CDK6
SYNONYMS
CDK 6 antibody;CDK6 antibody;CDK6_HUMAN antibody;Cell division protein kinase 6 antibody;Crk 2 antibody;Crk2 antibody;Cyclin dependent kinase 6 antibody;Cyclin-dependent kinase 6 antibody;MGC59692 antibody;p40 antibody;PLSTIRE antibody;Serine/threonine protein kinase PLSTIRE antibody;Serine/threonine-protein kinase PLSTIRE antibody;STQTL11 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
TISSUE SPECIFICITY
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
POST-TRANSLATIONAL MODIFICATION
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
SUBCELLULAR LOCATION
Cytoplasm, nucleus, Cell projection, Cytoskeleton.
FUNCTION
CDK6 protein contains a catalytic core composed of a serine/threonine domain.This protein also contains an ATP-binding pocket, inhibitory and activating phosphorylation sites, a PSTAIRE-like cyclin-binding domain and an activating T-loop motif. CDK6 is involved in a positive feedback loop that activates transcription factors through a reaction cascade. CDK6 is important for the control of G1 to S phase transition. However, in recent years, new evidence proved that the presence of CDK6 is not essential for proliferation in every cell type, the cell cycle has a complex circuitry of regulation and the role of CDK6 might be more important in certain cell types than in others, where CDK4 or CDK2 can act as protein kinases compensating its role.
CITATIONS
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Chen, X., Huang, Y., Gao, P., Lv, Y., Jia, D., Sun, K., Han, Y., Hu, H., Tang, Z., Ren, X., & Liu, M.
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells
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WB
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Zebrafish
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Citation
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Xin, H., Liu, Y., Chen, P., Yin, T., Wang, M., Liu, T., Wen, Z., & Cheng, Y.
CD155 promotes radioresistance and malignancy of esophageal cancer by regulating Hippo-YAP pathway
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WB
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Citation
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Natural small molecule triptonide inhibits lethal acute myeloid leukemia with FLT3-ITD mutation by targeting Hedgehog/FLT3 signaling
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WB
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Human
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Citation
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