Cdk6 Recombinant Rabbit Monoclonal Antibody [SD20-50] (ET1612-3)
Catalog# ET1612-3
Rabbit Monoclonal to Cdk6
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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WB
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Human
Lane 1: HeLa cell lysate
Lane 2: SK-OV-3 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-3) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cdk6 Recombinant Rabbit Monoclonal Antibody [SD20-50] (ET1612-3)
Immunogen
Synthetic peptide within human cdk6 aa 277-326 / 326.
Host
Rabbit
Positive Control
HeLa cell lysate, SK-OV-3 cell lysate, MCF7 cell lysate, K-562 cell lysate, Hela, SW480, 293, human colon carcinoma tissue, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD20-50
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cdk6
SYNONYMS
CDK 6 antibody;CDK6 antibody;CDK6_HUMAN antibody;Cell division protein kinase 6 antibody;Crk 2 antibody;Crk2 antibody;Cyclin dependent kinase 6 antibody;Cyclin-dependent kinase 6 antibody;MGC59692 antibody;p40 antibody;PLSTIRE antibody;Serine/threonine protein kinase PLSTIRE antibody;Serine/threonine-protein kinase PLSTIRE antibody;STQTL11 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
TISSUE SPECIFICITY
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
POST-TRANSLATIONAL MODIFICATION
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cell projection.
FUNCTION
Cell division protein kinase 6 (CDK6) is an enzyme encoded by the CDK6 gene.[5][6] It is regulated by cyclins, more specifically by Cyclin D proteins and Cyclin-dependent kinase inhibitor proteins.The protein encoded by this gene is a member of the cyclin-dependent kinase, (CDK) family, which includes CDK4. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression in the point of regulation named R or restriction point. This kinase is a catalytic subunit of the protein kinase complex, important for the G1 phase progression and G1/S transition of the cell cycle and the complex is composed also by an activating sub-unit; the cyclin D.The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors.This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor Retinoblastoma protein making CDK6 an important protein in cancer development.
CITATIONS
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Lei Tao,et al
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