Cdk4 Recombinant Rabbit Monoclonal Antibody [SD20-42] (ET1612-1)
Catalog# ET1612-1
Rabbit Monoclonal to Cdk4
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WB
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IF-Tissue
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IHC-P
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Human
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 30 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cdk4 Recombinant Rabbit Monoclonal Antibody [SD20-42] (ET1612-1)
Immunogen
Recombinant protein within mouse cdk4 aa 180-303.
Host
Rabbit
Positive Control
HeLa cell lysate, MCF7 cell lysate, human lung cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD20-42
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cdk4
SYNONYMS
Cdk 4 antibody;cdk4 antibody;CDK4 protein antibody;CDK4_HUMAN antibody;Cell division kinase 4 antibody;Cell division protein kinase 4 antibody;CMM 3 antibody;CMM3 antibody;Crk3 antibody;Cyclin dependent kinase 4 antibody;Cyclin-dependent kinase 4 antibody;Melanoma cutaneous malignant 3 antibody;MGC14458 antibody;p34 cdk4 antibody;PSK J3 antibody;PSK-J3 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
SUBCELLULAR LOCATION
Nucleus, Nucleus membrane, Cytoplasm.
FUNCTION
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
CITATIONS
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Hu, J., Cai, D., Zhao, Z., Zhong, G. C., & Gong, J.
Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway. Frontiers in oncology, 11, 659676.
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WB
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IHC
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Human
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Citation
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Overexpression of transcription factor 3 drives hepatocarcinoma development by enhancing cell proliferation via activating Wnt signaling pathway
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WB
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Human
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Citation
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Liu, Y., Wang, C., Cheng, P., Zhang, S., Zhou, W., Xu, Y., Xu, H., & Ji, G.
FHL1 Inhibits the Progression of Colorectal Cancer by Regulating the Wnt/β-Catenin Signaling Pathway. Journal of Cancer, 12(17), 5345–5354.
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WB
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Human
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Citation
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Wen, Q. L., Yi, H. Q., Yang, K., Yin, C. T., Yin, W. J., Xiang, F. Y., Bao, M., Shuai, J., Song, Y. W., Ge, M. H., & Zhu, X.
Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: Involvement of oxidative stress. Molecular and cellular endocrinology, 523, 111144.
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WB
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Human
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Citation
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