Cdk4 Mouse Monoclonal Antibody [5-C3-G3] (M1505-5)
Catalog# M1505-5
Mouse Monoclonal to Cdk4
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: Mouse lung tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 30 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1505-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Cdk4 Mouse Monoclonal Antibody [5-C3-G3] (M1505-5)
Immunogen
Recombinant protein within human cdk4 aa 80-303/303.
Host
Mouse
Positive Control
A549 cell lysate, 293T cell lysate, HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, Mouse lung tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Rat lung tissue lysate, HeLa, human breast cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
5-C3-G3
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG2c
TARGET
UNIPROT #
PROTEIN NAME
Cdk4
SYNONYMS
Cdk 4 antibody;cdk4 antibody;CDK4 protein antibody;CDK4_HUMAN antibody;Cell division kinase 4 antibody;Cell division protein kinase 4 antibody;CMM 3 antibody;CMM3 antibody;Crk3 antibody;Cyclin dependent kinase 4 antibody;Cyclin-dependent kinase 4 antibody;Melanoma cutaneous malignant 3 antibody;MGC14458 antibody;p34 cdk4 antibody;PSK J3 antibody;PSK-J3 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Nucleus membrane.
FUNCTION
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
CITATIONS
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Chen, L., Zhang, Y., Shu, X., Chen, Q., Wei, T., Wang, H., Wang, X., Wu, Q., Zhang, X., Liu, X., Zheng, S., Huang, L., Xiao, J., Jiang, C., Yang, B., Wang, Z., & Guo, X.
Proteasome regulation by reversible tyrosine phosphorylation at the membrane
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WB
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Mouse
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Citation
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