CDCA5 Recombinant Rabbit Monoclonal Antibody [JE59-54] (HA720045)
Catalog# HA720045
Rabbit Monoclonal to CDCA5
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CDCA5 on different lysates with Rabbit anti-CDCA5 antibody (HA720045) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: K-562 cell lysate
Lane 3: NCI-H441 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 35 kDa
Exposure time: 1 minute 39 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720045) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: K-562 cell lysate
Lane 3: NCI-H441 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 35 kDa
Exposure time: 1 minute 39 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720045) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.