Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Recombinant Rabbit monoclonal primary
CDC5L Recombinant Rabbit Monoclonal Antibody [JJ091-05] (ET1701-75)
Synthetic peptide within human cdc5l aa 320-370.
Jurkat, MCF-7, Hela, HepG2, NIH/3T3, 293T, SH-SY-5Y, human kidney tissue, mouse lung tissue, mouse kidney tissue, mouse stomach tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
CDC5 antibody; CDC5 cell division cycle 5-like (S. pombe) antibody; Cdc5 related protein antibody; CDC5-LIKE antibody; Cdc5-like protein antibody; Cdc5l antibody; CDC5L_HUMAN antibody; CEF1 antibody; Cell division cycle 5 S. pombe homolog of antibody; Cell division cycle 5-like protein antibody; dJ319D22.1 antibody; KIAA0432 antibody; PCDC5RP antibody; Pombe Cdc5 related protein antibody; Pombe Cdc5-related protein antibody
Belongs to the CEF1 family.
Ubiquitously expressed in both fetal and adult tissues.
Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Nucleus, Nucleus speckle, Cytoplasm.
Cdc5L (Cell division cycle 5-like protein, Pombe Cdc5-related protein) is a DNA-binding protein encoded by the human gene CDC5L. Cdc5L contains 2 HTH myb-type DNA-binding domains and may shuttle between cytoplasm and nucleus. It is involved in cell cycle control and may act as a transcription activator. CDC5L is a spliceosomal protein that is highly conserved across species. It is a member of a protein group that comprise the core of spliceosomal complexes and are essential for pre-mRNA splicing. Cdc5L is involved in the second catalytic step of pre-mRNA splicing, which involves cleavage at the 3' splice site and the ligation of the exons. This process releases the intact intron lariat. A chromosomal aberration involving Cdc5L is found in multicystic renal dysplasia. This aberration is caused by a translocation with USF-2.