CDC42EP1 Recombinant Rabbit Monoclonal Antibody [SD08-54] (ET1612-95)
Catalog# ET1612-95
Rabbit Monoclonal to CDC42EP1
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CDC42EP1 on different lysates with Rabbit anti-CDC42EP1 antibody (ET1612-95) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HUVEC cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HUVEC cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.