PRODUCT CODE: ET1612-94

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] (ET1612-94)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela              <br />
Lane 2: K562
  • Western blot analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela              <br />
Lane 2: K562
Western blot analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] (ET1612-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD85-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

92 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

Cdc27

SYNONYMS

ANAPC3 antibody; Anaphase Promoting Complex 3 antibody; Anaphase promoting complex protein 3 antibody; Anaphase Promoting Complex Subunit 3 antibody; Anaphase-promoting complex subunit 3 antibody; APC 3 antibody; APC3 antibody; Cdc 27 antibody; Cdc27 antibody; CDC27 homolog antibody; CDC27_HUMAN antibody; CDC27Hs antibody; Cell Division Cycle 27 antibody; Cell division cycle protein 27 homolog antibody; D0S1430E antibody; D17S978E antibody; H NUC antibody; H-NUC antibody; HNUC antibody; Nuc2 homolog antibody

SEQUENCE SIMILARITIES

Belongs to the APC3/CDC27 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

n the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.