Cdc25C Recombinant Rabbit Monoclonal Antibody [JE59-53] (HA722069)
Catalog# HA722069
Rabbit Monoclonal to Cdc25C
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WB
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IF-Cell
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IHC-P
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FC
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Human
Lane 1: 293T cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 58 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722069) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cdc25C Recombinant Rabbit Monoclonal Antibody [JE59-53] (HA722069)
Immunogen
Synthetic peptide within human cdc25c aa 400-473 (c terminal).
Host
Rabbit
Positive Control
293T cell lysate, Caco-2 cell lysate, HUVEC cell lysate, HT-29 cell lysate, HeLa, human breast cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE59-53
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:2,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cdc25C
SYNONYMS
CDC 25 antibody;Cdc 25C antibody;CDC25 antibody;CDC25C antibody;Cell division cycle 25 homolog C antibody;Cell division cycle 25C antibody;Cell division cycle 25C protein antibody;Dual specificity phosphatase Cdc25C antibody;M phase inducer phosphatase 3 antibody;M-phase inducer phosphatase 3 antibody;Mitosis inducer CDC25 antibody;MPIP3 antibody;MPIP3_HUMAN antibody;Phosphotyrosine phosphatase antibody;PPP1R60 antibody;protein phosphatase 1, regulatory subunit 60 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
CITATIONS
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Autophagy-dependent cell cycle arrest in esophageal cancer cells exposed to dihydroartemisinin
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WB
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Human
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Citation
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