Positive control:
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
CD99 Rabbit Polyclonal Antibody (ER1803-81)
Immunogen
Recombinant protein within human cd99 aa 1-185 / 185.
Host
Rabbit
Positive Control
SiHa, HepG2, rat kidney tissue, human esophagus tissue, human pancreas tissue, mouse testis tissue, 293T.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
25 kDa (predicted molecular weight: 19 kDa)
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
ICC:1:200
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD99
SYNONYMS
12E7 antibody; Antigen identified by monoclonal 12E7, Y homolog antibody; Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody; CD99 antibody; CD99 antigen antibody; CD99 molecule antibody; CD99_HUMAN antibody; Cell surface antigen 12E7 antibody; Cell surface antigen HBA 71 antibody; Cell surface antigen O13 antibody; E2 antigen antibody; HBA71 antibody; MIC 2X antibody; MIC 2Y antibody; MIC2 (monoclonal antibody 12E7) antibody; MIC2 antibody; MIC2X antibody; MIC2Y antibody; MSK5X antibody; Protein MIC2 antibody; Surface antigen MIC2 antibody; T cell surface glycoprotein E2 antibody; T-cell surface glycoprotein E2 antibody
SEQUENCE SIMILARITIES
Belongs to the CD99 family.
POST-TRANSLATIONAL MODIFICATION
Extensively O-glycosylated.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology.