CD99 Recombinant Rabbit Monoclonal Antibody [JF0991] (ET1702-35)
Catalog# ET1702-35
Rabbit Monoclonal to CD99
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U-937 cell lysate
Lane 4: MOLT-4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 30 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD99 Recombinant Rabbit Monoclonal Antibody [JF0991] (ET1702-35)
Immunogen
Recombinant protein within human cd99 aa 1-130 / 185.
Host
Rabbit
Positive Control
THP-1 cell lysate, Jurkat cell lysate, U-937 cell lysate, MOLT-4 cell lysate, Hela, PANC-1, PC-3M, human pancreas tissue, human uterus tissue, human tonsil tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0991
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 19 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD99
SYNONYMS
12E7 antibody;Antigen identified by monoclonal 12E7, Y homolog antibody;Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody;CD99 antibody;CD99 antigen antibody;CD99 molecule antibody;CD99_HUMAN antibody;Cell surface antigen 12E7 antibody;Cell surface antigen HBA 71 antibody;Cell surface antigen O13 antibody;E2 antigen antibody;HBA71 antibody;MIC 2X antibody;MIC 2Y antibody;MIC2 (monoclonal antibody;12E7) antibody;MIC2 antibody;MIC2X antibody;MIC2Y antibody;MSK5X antibody;Protein MIC2 antibody;Surface antigen MIC2 antibody;T cell surface glycoprotein E2 antibody;T-cell surface glycoprotein E2 antibody
SEQUENCE SIMILARITIES
Belongs to the CD99 family.
POST-TRANSLATIONAL MODIFICATION
Extensively O-glycosylated.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology.
CITATIONS
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Yang Yuling,et al
Exosome/antimicrobial peptide laden hydrogel wound dressings promote scarless wound healing through miR-21-5p-mediated multiple functions
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WB
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Human
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Mouse
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Citation
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