PRODUCT CODE: ET1702-35

CD99 Recombinant Rabbit Monoclonal Antibody [JF0991] (ET1702-35)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1,000 dilution.
  • Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1,000 dilution.
  • ICC staining CD99 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD99 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD99 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD99 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD99 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD99 Recombinant Rabbit Monoclonal Antibody [JF0991] (ET1702-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, PANC-1, Hela, PC-3M, human tonsil tissue, human pancreas tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0991

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD99

SYNONYMS

12E7 antibody; Antigen identified by monoclonal 12E7, Y homolog antibody; Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody; CD99 antibody; CD99 antigen antibody; CD99 molecule antibody; CD99_HUMAN antibody; Cell surface antigen 12E7 antibody; Cell surface antigen HBA 71 antibody; Cell surface antigen O13 antibody; E2 antigen antibody; HBA71 antibody; MIC 2X antibody; MIC 2Y antibody; MIC2 (monoclonal antibody; 12E7) antibody; MIC2 antibody; MIC2X antibody; MIC2Y antibody; MSK5X antibody; Protein MIC2 antibody; Surface antigen MIC2 antibody; T cell surface glycoprotein E2 antibody; T-cell surface glycoprotein E2 antibody

SEQUENCE SIMILARITIES

Belongs to the CD99 family.

POST-TRANSLATIONAL MODIFICATION

Extensively O-glycosylated.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology.