PRODUCT CODE: ER1902-56

CD95 Rabbit Polyclonal Antibody (ER1902-56)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of CD95 on Daudi cell  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-56, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD95 on Daudi cell  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-56, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of CD95 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-56, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD95 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-56, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD95 Rabbit Polyclonal Antibody (ER1902-56)

Immunogen

Synthetic peptide within c-terminal human cd95.

Host

Rabbit

Positive Control

Daudi cell lysates, A431.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD95

SYNONYMS

ALPS 1A antibody; ALPS1A antibody; APO 1 antibody; Apo 1 antigen antibody; APO 1 cell surface antigen antibody; Apo-1 antigen antibody; APO1 antibody; Apo1 antigen antibody; APO1 cell surface antigen antibody; Apoptosis antigen 1 antibody; Apoptosis mediating surface antigen FAS antibody; Apoptosis-mediating surface antigen FAS antibody; APT 1 antibody; APT1 antibody; CD 95 antibody; CD 95 antigen antibody; CD95 antibody; CD95 antigen antibody; Delta Fas antibody; Delta Fas/APO 1/CD95 antibody; Delta Fas/APO1/CD95 antibody; Fas (TNF receptor superfamily, member 6) antibody; FAS 1 antibody; FAS 827dupA antibody; Fas AMA antibody; Fas antibody; FAS Antigen antibody; Fas cell surface death receptor antibody; FAS1 antibody; FASLG receptor antibody; FASTM antibody; sFAS antibody; Surface antigen APO1 antibody; TNF receptor superfamily, member 6 antibody; TNFRSF 6 antibody; TNFRSF6 antibody; TNR6_HUMAN antibody; Tumor necrosis factor receptor superfamily member 6 antibody

TISSUE SPECIFICITY

Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane.; N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

SUBCELLULAR LOCATION

Cell membrane, Membrane, Secreted.

FUNCTION

Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno-surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity.