PRODUCT CODE: ET1608-46

CD90 Recombinant Rabbit Monoclonal Antibody [SU35-07] (ET1608-46)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Rat

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Western blot analysis of CD90 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD90 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CD90 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD90 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD90 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD90 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD90 Recombinant Rabbit Monoclonal Antibody [SU35-07] (ET1608-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung carcinoma tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU35-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

18 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD90

SYNONYMS

CD7 antibody; CD90 antibody; CD90 antigen antibody; CDw90 antibody; FLJ33325 antibody; MGC128895 antibody; T25 antibody; Theta antigen antibody; Thy 1 antibody; Thy 1 cell surface antigen antibody; Thy 1 membrane glycoprotein antibody; Thy 1 T cell antigen antibody; Thy 1.2 antibody; Thy-1 antigen antibody; Thy-1 membrane glycoprotein antibody; Thy1 antibody; Thy1 antigen antibody; Thy1 T cell antigen antibody; Thy1.1 antibody; Thy1.2 antibody; THY1_HUMAN antibody; Thymus cell antigen 1, theta antibody

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a "cluster of differentiation" (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant.