PRODUCT CODE: 0108-7

CD8 Rabbit Polyclonal Antibody (0108-7)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Mouse

Western blot analysis on mouse Thymus using anti-CD8  polyclonal antibody
  • Western blot analysis on mouse Thymus using anti-CD8  polyclonal antibody
Western blot analysis on mouse Thymus using anti-CD8 polyclonal antibody

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD8 Rabbit Polyclonal Antibody (0108-7)

Immunogen

Synthetic peptide within mouse cd8 aa30-70.

Host

Rabbit

Positive Control

Mouse thymus tisue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

32-36kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

CD8

SYNONYMS

CD8 antibody; CD8 antigen, alpha polypeptide (p32) antibody; CD8a antibody; CD8a antigen antibody; CD8a molecule antibody; CD8A_HUMAN antibody; Leu2 antibody; Leu2 T lymphocyte antigen antibody; MAL antibody; OKT8 T cell antigen antibody; p32 antibody; T cell antigen Leu2 antibody; T cell co receptor antibody; T-cell surface glycoprotein CD8 alpha chain antibody; T-lymphocyte differentiation antigen T8/Leu-2 antibody; T8 T cell antigen antibody

POST-TRANSLATIONAL MODIFICATION

Palmitoylated, but association with CD8B seems to be more important for the enrichment of CdD8A in lipid rafts.; Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 is thought to play a role in the process of T-cell mediated killing.