CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40] (ET1703-41)
Catalog# ET1703-41
Rabbit Monoclonal to CD73
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WB
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IHC-P
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IF-Tissue
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Human
Lane 1: HT-29 cell lysate
Lane 2: U-87 MG cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 70 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40] (ET1703-41)
Immunogen
Synthetic peptide within human cd73 aa 491-536 / 574.
Host
Rabbit
Positive Control
HT-29 cell lysate, U-87 MG cell lysate, HepG2 cell lysate, A375 cell lysate, human endometrium tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-40
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200-1:1,000
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IF-Tissue:1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 63 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD73
SYNONYMS
5' NT antibody;5' nucleotidase (CD73) antibody;5' nucleotidase precursor antibody;5' nucleotidase, ecto antibody;5' nucleotidase, ecto (CD73) antibody;5'-NT antibody;5'-nucleotidase antibody;5NTD_HUMAN antibody;CD73 antibody;CD73 antigen antibody;E5NT antibody;Ecto 5' nucleotidase antibody;Ecto-5'-nucleotidase antibody;eN antibody;eNT antibody;NT antibody;NT5 antibody;NT5E antibody;NTE antibody;Purine 5 Prime Nucleotidase antibody
SEQUENCE SIMILARITIES
Belongs to the 5'-nucleotidase family.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
CITATIONS
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Yang Fan,et al
Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation
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WB
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Mouse
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Citation
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