PRODUCT CODE: ET1703-41

CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40] (ET1703-41)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

-
+
Western blot analysis of CD73 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD73 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD73 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD73 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD73 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD73 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD73 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40] (ET1703-41)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431 cell lysates, human placenta tissue lysates, human tonsil tissue, human uterus tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-40

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD73

SYNONYMS

5' NT antibody; 5' nucleotidase (CD73) antibody; 5' nucleotidase precursor antibody; 5' nucleotidase, ecto antibody; 5' nucleotidase, ecto (CD73) antibody; 5'-NT antibody; 5'-nucleotidase antibody; 5NTD_HUMAN antibody; CD73 antibody; CD73 antigen antibody; E5NT antibody; Ecto 5' nucleotidase antibody; Ecto-5'-nucleotidase antibody; eN antibody; eNT antibody; NT antibody; NT5 antibody; NT5E antibody; NTE antibody; Purine 5 Prime Nucleotidase antibody

SEQUENCE SIMILARITIES

Belongs to the 5'-nucleotidase family.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.