CD7 Recombinant Rabbit Monoclonal Antibody [JE57-72] (HA720023)
Catalog# HA720023
Rabbit Monoclonal to CD7
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD7 on different lysates with Rabbit anti-CD7 antibody (HA720023) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate (negative)
Lane 3: MOLT-4 cell lysate
Lane 4: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 35-40 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720023) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD7 on different lysates with Rabbit anti-CD7 antibody (HA720023) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate (negative)
Lane 3: MOLT-4 cell lysate
Lane 4: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 35-40 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720023) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.