Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Human lung tissue lysate
Applications
-
WB
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
CD68 Rabbit Polyclonal Antibody (ER1902-74)
Immunogen
Synthetic peptide within human cd68 aa 50-120.
Host
Rabbit
Positive Control
MCF-7 cell lysate, human lung tissue lysate, THP-1.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
Predicted band size 37 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD68
SYNONYMS
CD 68 antibody; CD68 antibody; CD68 antigen antibody; CD68 molecule antibody; CD68_HUMAN antibody; DKFZp686M18236 antibody; gp11 antibody; Gp110 antibody; LAMP4 antibody; Macrophage antigen CD68 (microsialin) antibody; MACROPHAGE ANTIGEN CD68 antibody; Macrosialin antibody; SCARD1 antibody; Scavenger receptor class D member 1 antibody
SEQUENCE SIMILARITIES
Belongs to the LAMP family.
TISSUE SPECIFICITY
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
POST-TRANSLATIONAL MODIFICATION
N- and O-glycosylated.
SUBCELLULAR LOCATION
Cell membrane, Endosome, Lysosome, Membrane
FUNCTION
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.