CD68 Recombinant Mouse Monoclonal Antibody [PDM0-13] (HA601115)
Catalog# HA601115
Mouse Monoclonal to CD68
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IHC-P
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IF-Cell
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FC
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mIHC
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IF-Tissue
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Human
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601115) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
CD68 Recombinant Mouse Monoclonal Antibody [PDM0-13] (HA601115)
Immunogen
Recombinant protein within human cd68 aa 1-354.
Host
Mouse
Positive Control
Human spleen tissue, human diffuse large B-cell lymphoma tissue, human tonsil tissue, human liver tissue, THP-1, human non-small cell lung cancer, human cervical cancer.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PDM0-13
RRID
APPLICATION DILUTION
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IHC-P
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1:200-1:4,000
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IF-Cell
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1:100
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FC
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1:500-1:1,000
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mIHC
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1:2,000
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IF-Tissue
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1:50-1:800
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
CD68
SYNONYMS
CD 68 antibody;CD68 antibody;CD68 antigen antibody;CD68 molecule antibody;CD68_HUMAN antibody;DKFZp686M18236 antibody;gp11 antibody;Gp110 antibody;LAMP4 antibody;Macrophage antigen CD68 (microsialin) antibody;MACROPHAGE ANTIGEN CD68 antibody;Macrosialin antibody;SCARD1 antibody;Scavenger receptor class D member 1 antibody
SUBCELLULAR LOCATION
Cell membrane. Endosome membrane, Lysosome membrane.
FUNCTION
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Human CD68 is a transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a molecular weight of 110 kD. Its primary sequence consists of 354 amino acids with predicted molecular weight of 37.4 kD if it were not glycosylated. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells and myocytes. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. Anti-CD68 monoclonal antibodies that react with tissues of rodent and other species include ED1, FA-11, KP1 (a.k.a. C68/684), 6A326, 6F3, 12E2, 10B1909, and SPM130. Monoclonals that react with humans include, Ki-M7, PG-M1, 514H12, ABM53F5, 3F7C6, 3F7D3, Y1/82A, EPR20545, CDLA68-1, LAMP4-824.
CITATIONS
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Fusobacterium nucleatum Facilitates M2 Macrophage Polarization and Colorectal Carcinoma Progression by Activating TLR4/NF-κB/S100A9 Cascade
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IHC
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Human
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Citation
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Xiaoxiao Li,et al
Cholesterol overload in macrophages drives metabolic dysfunction-associated steatohepatitis via inhibiting 7-dehydrocholesterol reductase in mice
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IF
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Mouse
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Citation
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