Positive control:
Lane 1: A431 cell lysate
Lane 2: THP-1 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
CD68 Mouse Monoclonal Antibody [A3C3] (EM1901-94)
Immunogen
Synthetic peptide within human cd68 aa 320-354.
Host
Mouse
Positive Control
A431 cell lysate, THP-1 cell lysate, human tonsil tissue, human spleen tissue, human lung cancer tissue, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3C3
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
Predicted band size: 37 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:1,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD68
SYNONYMS
CD 68 antibody; CD68 antibody; CD68 antigen antibody; CD68 molecule antibody; CD68_HUMAN antibody; DKFZp686M18236 antibody; gp11 antibody; Gp110 antibody; LAMP4 antibody; Macrophage antigen CD68 (microsialin) antibody; MACROPHAGE ANTIGEN CD68 antibody; Macrosialin antibody; SCARD1 antibody; Scavenger receptor class D member 1 antibody
SEQUENCE SIMILARITIES
Belongs to the LAMP family.
TISSUE SPECIFICITY
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
POST-TRANSLATIONAL MODIFICATION
N- and O-glycosylated.
SUBCELLULAR LOCATION
Cell membrane. Endosome membrane, lysosome membrane.
FUNCTION
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Human CD68 is a transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a molecular weight of 110 kD. Its primary sequence consists of 354 amino acids with predicted molecular weight of 37.4 kD if it were not glycosylated. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells and myocytes. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. Anti-CD68 monoclonal antibodies that react with tissues of rodent and other species include ED1, FA-11, KP1 (a.k.a. C68/684), 6A326, 6F3, 12E2, 10B1909, and SPM130. Monoclonals that react with humans include, Ki-M7, PG-M1, 514H12, ABM53F5, 3F7C6, 3F7D3, Y1/82A, EPR20545, CDLA68-1, LAMP4-824.