CD63 Recombinant Rabbit Monoclonal Antibody [SY21-02] (ET1607-2)
Catalog# ET1607-2
Rabbit Monoclonal to CD63
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WB
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IHC-P
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Human
Lane 1: SK-MEL-28 cell lysate
Lane 2: U-87 MG cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30~60 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD63 Recombinant Rabbit Monoclonal Antibody [SY21-02] (ET1607-2)
Immunogen
Recombinant protein within human cd63 aa 88-216 / 238.
Host
Rabbit
Positive Control
SK-MEL-28 cell lysate, U-87 MG cell lysate, human lung carcinoma tissue, human tonsil tissue, human spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY21-02
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD63
SYNONYMS
Lysosomal associated membrane protein 3 antibody;CD 63 antibody;CD63 antibody;CD63 antigen (melanoma 1 antigen) antibody;CD63 antigen antibody;CD63 antigen melanoma 1 antigen antibody;CD63 molecule antibody;CD63_HUMAN antibody;gp55 antibody;Granulophysin antibody;LAMP 3 antibody;LAMP-3 antibody;LAMP3 antibody;LIMP antibody;Lysosomal-associated membrane protein 3 antibody;Lysosome associated membrane glycoprotein 3 antibody;Mast cell antigen AD1 antibody;ME491 antibody;Melanoma 1 antigen antibody;Melanoma associated antigen ME491 antibody;Melanoma associated antigen MLA1 antibody;Melanoma-associated antigen ME491 antibody;MGC72893 antibody;MLA 1 antibody;MLA1 antibody;NGA antibody;Ocular melanoma associated antigen antibody;Ocular melanoma-associated antigen antibody;OMA81H antibody;PTLGP40 antibody;Tetraspanin 30 antibody;Tetraspanin-30 antibody;Tspan 30 antibody;Tspan-30 antibody;TSPAN30 antibody
SEQUENCE SIMILARITIES
Belongs to the tetraspanin (TM4SF) family.
TISSUE SPECIFICITY
Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
POST-TRANSLATIONAL MODIFICATION
Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
SUBCELLULAR LOCATION
Lysosome membrane, Cell membrane, extracellular exosome, Late endosome membrane, multivesicular body, Melanosome, Cell surface.
FUNCTION
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.
CITATIONS
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IF
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c cancer cell lines BxPC-3
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PANC-1
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and AsPC-1
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Citation
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Lin, Y., Li, Y., Chen, P., Zhang, Y., Sun, J., Sun, X., Li, J., Jin, J., Xue, J., Zheng, J., Jiang, X. C., Chen, C., Li, X., Wu, Y., Zhao, W., Liu, J., Ye, X., Zhang, R., Gao, J., & Zhang, D.
Exosome-Based Regimen Rescues Endometrial Fibrosis in Intrauterine Adhesions Via Targeting Clinical Fibrosis Biomarkers
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WB
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Human
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XiMing Xu
Exosomes derived from liver cancer cells reprogram biological behaviors of LO2 cells by transferring Linc-ROR
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WB
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The human hepatocellular carcinoma cell lines
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Shen, M., Fan, X., Shen, Y., Wang, X., Wu, R., Wang, Y., Huang, C., Zhao, S., Zheng, Y., Men, R., Luo, X., & Yang, L.
Myeloid-derived suppressor cells ameliorate liver mitochondrial damage to protect against autoimmune hepatitis by releasing small extracellular vesicles
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WB
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Human
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Citation
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Liming Zhao,et al
Clinical significance of a new early diagnostic model for bladder cancer based on genome-wide microarray profiling of serum exosomal lncRNAs
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WB
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Human
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Citation
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