PRODUCT CODE: ET1703-49

CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95] (ET1703-49)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.<br />
Positive control: <br />
Line1: HUVEC         <br />
Line2: Human placenta
  • Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.<br />
Positive control: <br />
Line1: HUVEC         <br />
Line2: Human placenta
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. Counter stained with hematoxylin.
Western blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution.
Positive control:
Line1: HUVEC
Line2: Human placenta

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95] (ET1703-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, human placenta, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-95

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD62P

SYNONYMS

Antigen CD62 antibody; CD62 antibody; CD62 antigen-like family member P antibody; CD62P antibody; GMP 140 antibody; GMP-140 antibody; GMP140 antibody; GMRP antibody; Granule membrane protein 140 antibody; Granulocyte membrane protein antibody; GRMP antibody; LECAM 3 antibody; LECAM3 antibody; Leukocyte endothelial cell adhesion molecule 3 antibody; Leukocyte-endothelial cell adhesion molecule 3 antibody; LYAM3_HUMAN antibody; P Selectin antibody; P-selectin antibody; PADGEM antibody; Platelet activation dependent granule-external membrane protein antibody; Platelet alpha granule membrane protein antibody; PSEL antibody; Selectin P (granule membrane protein 140kDa, antigen CD62) antibody; SELP antibody

SEQUENCE SIMILARITIES

Belongs to the selectin/LECAM family.

TISSUE SPECIFICITY

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes.