PRODUCT CODE: ER1802-46

CD55 Rabbit Polyclonal Antibody (ER1802-46)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD55 on human plasma using anti-CD55 antibody at 1/1,000
  • Western blot analysis of CD55 on human plasma using anti-CD55 antibody at 1/1,000
  • Western blot analysis of CD55 on Hela and PC-3M cell lysates using anti-CD55 antibody at 1/500 dilution.
  • ICC staining CD55 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD55 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD55 antibody. Counter stained with hematoxylin.
Western blot analysis of CD55 on human plasma using anti-CD55 antibody at 1/1,000

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD55 Rabbit Polyclonal Antibody (ER1802-46)

Immunogen

Recombinant protein within human cd55 aa 130-381.

Host

Rabbit

Positive Control

Hela and PC-3M cell lysates, human plasma, human colon cancer tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

42/49/60 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD55

SYNONYMS

CD 55 antibody; CD55 antibody; CD55 antigen antibody; CD55 Cromer blood group system antibody; CD55 molecule (Cromer blood group) antibody; CD55 molecule antibody; CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody; Cd55a antibody; Complement decay accelerating factor antibody; Complement decay-accelerating factor antibody; Complement decay-accelerating factor, GPI-anchored antibody; CR antibody; CROM antibody; Cromer Blood Group antigen antibody; Cromer blood group system antibody; DAF antibody; Daf-GPI antibody; DAF_HUMAN antibody; Daf1 antibody; Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody; Decay accelarating factor 1, isoform CRA_a antibody; Decay accelerating factor (GPI-form) antibody; Decay Accelerating Factor for Complement antibody; Decay accelerating factor GPI-form antibody; Decay accelerating factor soluble-form antibody; GPI-DAF antibody; TC antibody

SEQUENCE SIMILARITIES

Belongs to the receptors of complement activation (RCA) family.

TISSUE SPECIFICITY

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

POST-TRANSLATIONAL MODIFICATION

The Ser/Thr-rich domain is heavily O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane. Secreted.

FUNCTION

CD55, also called decay accelerating factor (DAF), is a GPI-anchored single chain glycoprotein. CD55 may play a role in protecting cells from complement-mediated lysis by preventing the amplification steps of the complement cascade. CD55 functions to prevent the assembly of C3 convertase or to accelerate the disassembly of preformed convertase, which blocks formation of the membrane attack complex. CD55 is expressed on cells in contact with serum, including hematopoietic and many non-hematopoietic cells.