CD46 Recombinant Rabbit Monoclonal Antibody [JB25-49] (ET7107-94)
Catalog# ET7107-94
Rabbit Monoclonal to CD46
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WB
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IF-Cell
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IHC-P
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Human
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Rat
Western blot analysis of CD46 on different lysates with Rabbit anti-CD46 antibody (ET7107-94) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD46 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 55-70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-94) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD46 Recombinant Rabbit Monoclonal Antibody [JB25-49] (ET7107-94)
Immunogen
Synthetic peptide within human cd46 aa 31-80 / 392.
Host
Rabbit
Positive Control
SiHa cell lysate, PC-3M cell lysate, PC-3M, HUVEC, human colon carcinoma tissue, human colon tissue, human breast tissue, human placenta tissue, rat kidney tissue, human prostate tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB25-49
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:800
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 44 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD46
SYNONYMS
AHUS2 antibody;Antigen defined by monoclonal antibody TRA 2 10 antibody;Antigen identified by monoclonal antibody TRA 2 10 antibody;CD46 antibody;CD46 antigen antibody;CD46 antigen complement regulatory protein antibody;CD46 molecule antibody;CD46 molecule complement regulatory protein antibody;Complement membrane cofactor protein antibody;MCP antibody;MCP_HUMAN antibody;Measles virus receptor antibody;membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody;Membrane cofactor protein antibody;MGC26544 antibody;MIC10 antibody;TLX antibody;TRA2.10 antibody;Trophoblast leucocyte common antigen antibody;Trophoblast leukocyte common antigen antibody;Trophoblast lymphocyte cross reactive antigen antibody
TISSUE SPECIFICITY
Expressed by all cells except erythrocytes.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.; Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.; In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.
SUBCELLULAR LOCATION
Acrosome inner membrane.
FUNCTION
CD46, also called membrane cofactor protein (MCP), is a transmembrane glycoprotein that exists as a non-disulfide-linked dimer. CD46 regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. CD46 is a cofactor that binds to C3b and C4b, allowing their degradation by a plasma serine protease called factor I. This function resides in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is widely distributed on thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, platelets, endothelial cells, epithelial cells, fibroblasts, placenta and sperm, but not on erythrocytes. It is the major high affinity receptor for measles virus and human herpes virus. Mouse cells ubiquitously express CRRY, which is a functional ortholog of human decay-accelerating factor (DAF; CD55) and membrane cofactor protein (MCP; CD46).
CITATIONS
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Chen, L., Zhang, Y., Shu, X., Chen, Q., Wei, T., Wang, H., Wang, X., Wu, Q., Zhang, X., Liu, X., Zheng, S., Huang, L., Xiao, J., Jiang, C., Yang, B., Wang, Z., & Guo, X.
Proteasome regulation by reversible tyrosine phosphorylation at the membrane
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WB
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Mouse
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Citation
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