PRODUCT CODE: ER1902-02

CD45 Rabbit Polyclonal Antibody (ER1902-02)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of CD45 on Jurkat cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD45 Rabbit Polyclonal Antibody (ER1902-02)

Immunogen

Synthetic peptide within human cd45 aa 1-60.

Host

Rabbit

Positive Control

U937 cell lysates, Jurkat cell lysates, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size 147 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD45

SYNONYMS

B220 antibody; CD 45 antibody; CD45 antibody; CD45 antigen antibody; CD45R antibody; GP180 antibody; L-CA antibody; LCA antibody; Leukocyte common antigen antibody; loc antibody; Ly-5 antibody; LY5 antibody; Ly5, homolog of antibody; Lyt-4 antibody; OTTHUMP00000033813 antibody; OTTHUMP00000033816 antibody; OTTHUMP00000033817 antibody; OTTHUMP00000038574 antibody; Protein tyrosine phosphatase receptor type c polypeptide antibody; Protein tyrosine phosphatase, receptor type C antibody; protein tyrosine phosphatase, receptor type, C antibody; Protein tyrosine phosphatase, receptor type, c polypeptide antibody; Ptprc antibody; PTPRC_HUMAN antibody; Receptor-type tyrosine-protein phosphatase C antibody; T200 antibody; T200 glycoprotein antibody; T200 leukocyte common antigen antibody

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

TISSUE SPECIFICITY

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

DEVELOPMENTAL STAGE

Isoform 1: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 2: During T-cell development, expressed at low levels at the CD3-CD4-CD8- and CD3-CD4+CD8- stages but up-regulated at the CD3+CD4+CD8+ and CD3+CD4+CD8- stages. Isoform 3: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 5: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 7: Consistently expressed at high levels at all stages of T-cell development.

POST-TRANSLATIONAL MODIFICATION

Heavily N- and O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane, membrane raft.

FUNCTION

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.