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PRODUCT CODE: ER1803-26

CD42b Rabbit Polyclonal Antibody (ER1803-26)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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ICC staining CD42b in Jurkat cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD42b in Jurkat cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD42b antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD42b antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ICC staining CD42b in Jurkat cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD42b Rabbit Polyclonal Antibody (ER1803-26)

Immunogen

Recombinant protein within human cd42b aa 117-328 / 652.

Host

Rabbit

Positive Control

Jurkat, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

71 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

P07359

PROTEIN NAME

CD42b

SYNONYMS

Antigen CD42b alpha antibody; BDPLT1 antibody; BDPLT3 antibody; BSS antibody; CD 42b antibody; CD42b alpha antibody; CD42B antibody; CD42b antigen antibody; DBPLT3 antibody; GLYCOCALICIN antibody; Glycoprotein Ib (platelet) alpha polypeptide antibody; Glycoprotein Ibalpha antibody; GP Ib alpha antibody; GP1B antibody; GP1BA antibody; GPIb alpha antibody; GPIbA antibody; MGC34595 antibody; Platelet glycoprotein Ib alpha chain antibody; Platelet glycoprotein Ib alpha polypeptide antibody; Platelet membrane glycoprotein 1b alpha subunit antibody; VWDP antibody

POST-TRANSLATIONAL MODIFICATION

Glycocalicin is the product of a proteolytic cleavage/shedding, catalyzed by ADAM17, which releases most of the extracellular domain. Binding sites for vWF and thrombin are in this part of the protein.

SUBCELLULAR LOCATION

Membrane. Secreted.

FUNCTION

CD42a is a single-chain membrane glycoprotein that forms a noncovalent complex with CD42b. CD42b, also known as glycoprotein Ib a (GPIb a) is a membrane glycoprotein that is composed of a and b chains. The CD42b b chain is also designated CD42c, and is expressed on platelets and megakaryocytes. CD42a and CD42b are also present on platelets and mega-karyocytes, and the complex is a major component of the platelet surface. The complex acts as a receptor for von Willebrand's factor and as a von Willebrand's factor-dependent adhesion receptor.