PRODUCT CODE: ER1803-54

CD40 Rabbit Polyclonal Antibody (ER1803-54)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.
  • Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.
  • ICC staining CD40 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD40 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD40 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD40 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD40 Rabbit Polyclonal Antibody (ER1803-54)

Immunogen

Recombinant protein within human cd40 aa 1-213 / 277.

Host

Rabbit

Positive Control

Daudi, MG-63, SiHa, rat spleen tissue, human tonsil tissue, SKOV-3.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 31kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD40

SYNONYMS

AI326936 antibody; B cell associated molecule CD40 antibody; B cell surface antigen CD40 antibody; B cell-associated molecule antibody; B-cell surface antigen CD40 antibody; Bp50 antibody; CD 40 antibody; CD40 antibody; CD40 antigen (TNF receptor superfamily member 5) antibody; CD40 antigen antibody; CD40 molecule antibody; CD40 molecule, TNF receptor superfamily member 5 antibody; CD40 protein antibody; CD40 type II isoform antibody; CD40L receptor antibody; CDw40 antibody; GP39 antibody; HIGM1 antibody; IGM antibody; IMD3 antibody; MGC9013 antibody; Nerve growth factor receptor related B lymphocyte activation molecule antibody; OTTHUMP00000031699 antibody; OTTHUMP00000031700 antibody; p50 antibody; T-BAM antibody; TBAM antibody; TNF receptor superfamily member 5 antibody; TNFRSF5 antibody; TNR5_HUMAN antibody; TRAP antibody; Tumor necrosis factor receptor superfamily , member 5 antibody; Tumor necrosis factor receptor superfamily member 5 antibody; Tumor necrosis factor receptor superfamily member 5 precursor antibody; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody

TISSUE SPECIFICITY

B-cells and in primary carcinomas.

SUBCELLULAR LOCATION

Plasma membrane. Secreted.

FUNCTION

Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. A receptor ligand pair central to the trans-mission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif.