PRODUCT CODE: EM1901-40

CD40 Mouse Monoclonal Antibody [A1E6] (EM1901-40)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of CD40 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD40 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of CD40 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD40 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD40 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of CD40 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD40 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD40 Mouse Monoclonal Antibody [A1E6] (EM1901-40)

Immunogen

Recombinant protein within human cd40 aa 1-250.

Host

Mouse

Positive Control

Daudi cell lysates, MG-63, SiHa, SKOV-3, Daudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A1E6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • ICC:1:50-1:100

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD40

SYNONYMS

AI326936 antibody; B cell associated molecule CD40 antibody; B cell surface antigen CD40 antibody; B cell-associated molecule antibody; B-cell surface antigen CD40 antibody; Bp50 antibody; CD 40 antibody; CD40 antibody; CD40 antigen (TNF receptor superfamily member 5) antibody; CD40 antigen antibody; CD40 molecule antibody; CD40 molecule, TNF receptor superfamily member 5 antibody; CD40 protein antibody; CD40 type II isoform antibody; CD40L receptor antibody; CDw40 antibody; GP39 antibody; HIGM1 antibody; IGM antibody; IMD3 antibody; MGC9013 antibody; Nerve growth factor receptor related B lymphocyte activation molecule antibody; OTTHUMP00000031699 antibody; OTTHUMP00000031700 antibody; p50 antibody; T-BAM antibody; TBAM antibody; TNF receptor superfamily member 5 antibody; TNFRSF5 antibody; TNR5_HUMAN antibody; TRAP antibody; Tumor necrosis factor receptor superfamily , member 5 antibody; Tumor necrosis factor receptor superfamily member 5 antibody; Tumor necrosis factor receptor superfamily member 5 precursor antibody; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody

TISSUE SPECIFICITY

B-cells and in primary carcinomas.

SUBCELLULAR LOCATION

Cell membrane, secreted.

FUNCTION

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.