PRODUCT CODE: 0108-6

CD4 Rabbit Polyclonal Antibody (0108-6)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
  • Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
  • ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD4 Rabbit Polyclonal Antibody (0108-6)

Immunogen

Synthetic peptide within mouse cd4 aa 195-244 / 457.

Host

Rabbit

Positive Control

Mouse brain tissue, LOVO, Raji.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

51kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD4

SYNONYMS

CD 4 antibody; CD4 (L3T4) antibody; CD4 antibody; CD4 antigen (p55) antibody; CD4 antigen antibody; CD4 molecule antibody; CD4 receptor antibody; CD4+ Lymphocyte deficiency, included antibody; CD4_HUMAN antibody; CD4mut antibody; L3T4 antibody; Leu3 antibody; Ly-4 antibody; Lymphocyte antigen CD4 antibody; MGC165891 antibody; OTTHUMP00000238897 antibody; p55 antibody; T cell antigen T4 antibody; T cell antigen T4/LEU3 antibody; T cell differentiation antigen L3T4 antibody; T cell OKT4 deficiency, included antibody; T cell surface antigen T4/Leu 3 antibody; T cell surface antigen T4/Leu3 antibody; T cell surface glycoprotein CD4 antibody; T-cell surface antigen T4/Leu-3 antibody; T-cell surface glycoprotein CD4 antibody; W3/25 antibody; W3/25 antigen antibody

TISSUE SPECIFICITY

Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.; Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.

SUBCELLULAR LOCATION

Cell Membrane

FUNCTION

The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120.