PRODUCT CODE: ET1609-52

CD4 Recombinant Rabbit Monoclonal Antibody [ST0488] (ET1609-52)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: U937 cell lysate<br />
Lane 2: THP-1 cell lysate<br />
Lane 3: human thymus tissue lysate
  • Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: U937 cell lysate<br />
Lane 2: THP-1 cell lysate<br />
Lane 3: human thymus tissue lysate
  • ICC staining of CD4 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD4 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-52, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-52, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: U937 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: human thymus tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD4 Recombinant Rabbit Monoclonal Antibody [ST0488] (ET1609-52)

Immunogen

Recombinant protein within human cd4 aa 196-416 / 458.

Host

Rabbit

Positive Control

Human thymus tissue lysates, A549, CRC, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST0488

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD4

SYNONYMS

CD 4 antibody; CD4 (L3T4) antibody; CD4 antibody; CD4 antigen (p55) antibody; CD4 antigen antibody; CD4 molecule antibody; CD4 receptor antibody; CD4+ Lymphocyte deficiency, included antibody; CD4_HUMAN antibody; CD4mut antibody; L3T4 antibody; Leu3 antibody; Ly-4 antibody; Lymphocyte antigen CD4 antibody; MGC165891 antibody; OTTHUMP00000238897 antibody; p55 antibody; T cell antigen T4 antibody; T cell antigen T4/LEU3 antibody; T cell differentiation antigen L3T4 antibody; T cell OKT4 deficiency, included antibody; T cell surface antigen T4/Leu 3 antibody; T cell surface antigen T4/Leu3 antibody; T cell surface glycoprotein CD4 antibody; T-cell surface antigen T4/Leu-3 antibody; T-cell surface glycoprotein CD4 antibody; W3/25 antibody; W3/25 antigen antibody

TISSUE SPECIFICITY

Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.; Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV.

CITATIONS

  • Ming Zhang,Kangshou Yao

    Proteomic Analyses Reveal Common Promiscuous Patterns of Cell Surface Proteins on Human Embryonic Stem Cells and Sperms

  • Ming Zhang

    Global Expression of Cell Surface Proteins in Embryonic Stem Cells