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PRODUCT CODE: ER1802-7

CD36 Rabbit Polyclonal Antibody (ER1802-7)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining CD36 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD36 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD36 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD36 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CD36 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-CD36 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD36 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of THP-1 cells with CD36 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining CD36 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD36 Rabbit Polyclonal Antibody (ER1802-7)

Immunogen

Synthetic peptide within human cd36 aa 55-117.

Host

Rabbit

Positive Control

HepG2, HUVEC, SH-SY5Y, rat heart tissue, mouse small intestine tissue, mouse heart tissue, THP-1.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

PURIFICATION

Immunogen affinity purified.

MOLECULAR WEIGHT

Predicted band size: 53 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P16671

PROTEIN NAME

CD36

SYNONYMS

Adipocyte membrane protein antibody;BDPLT10 antibody;CD36 antibody;CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody;CD36 antigen antibody;CD36 molecule (thrombospondin receptor) antibody;CD36 molecule antibody;CD36_HUMAN antibody;CHDS7 antibody;Cluster determinant 36 antibody;Collagen receptor, platelet antibody;FAT antibody;Fatty acid translocase antibody;Fatty acid transport protein antibody;Glycoprotein IIIb antibody;GP IIIb antibody;GP3B antibody;GP4 antibody;GPIIIB antibody;GPIV antibody;Leukocyte differentiation antigen CD36 antibody;MGC108510 antibody;MGC91634 antibody;PAS 4 protein antibody;PAS IV antibody;PAS-4 antibody;PASIV antibody;Platelet collagen receptor antibody;Platelet glycoprotein 4 antibody;Platelet glycoprotein IV antibody;scarb3 antibody;Scavenger receptor class B member 3 antibody;Thrombospondin receptor antibody

SEQUENCE SIMILARITIES

Belongs to the CD36 family.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated and O-glycosylated with a ratio of 2:1.; Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome. Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids.

SUBCELLULAR LOCATION

Cell membrane. Golgi apparatus.

FUNCTION

CD36 (collagen type I receptor, thrombospondin receptor, FAT, GP4, GP3B, GPIV, PASIV, SCARB3) is a membrane glycoprotein on platelets, monocytes and umbilical vein endothelial cells. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. CD36 plays a key role in both phagocytosis and lipid recycling, for constant production of mature spermatozoa. Mutations in this gene cause platelet glycoprotein deficiency. Three alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. Thrombospondins are widely distributed proteins that influence a variety of adhesive processes and CD36 may have important functions as a cell adhesion molecule.