CD36 Recombinant Rabbit Monoclonal Antibody [JJ2005] (ET1701-24)
Catalog# ET1701-24
Rabbit Monoclonal to CD36
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: Mouse white adipose tissue lysate
Lane 2: Mouse brown adipose tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 80 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD36 Recombinant Rabbit Monoclonal Antibody [JJ2005] (ET1701-24)
Immunogen
Synthetic peptide within human cd36 aa 360-373 / 472.
Host
Rabbit
Positive Control
Human heart tissue lysate, Mouse heart tissue lysate, THP-1, THP-1 cells treated with 100ng/mL PMA for 72 hours, human spleen tissue, mouse spleen tissue, mouse heart tissue, mouse lung tissue, mouse stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ2005
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD36
SYNONYMS
Adipocyte membrane protein antibody;BDPLT10 antibody;CD36 antibody;CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody;CD36 antigen antibody;CD36 molecule (thrombospondin receptor) antibody;CD36 molecule antibody;CD36_HUMAN antibody;CHDS7 antibody;Cluster determinant 36 antibody;Collagen receptor, platelet antibody;FAT antibody;Fatty acid translocase antibody;Fatty acid transport protein antibody;Glycoprotein IIIb antibody;GP IIIb antibody;GP3B antibody;GP4 antibody;GPIIIB antibody;GPIV antibody;Leukocyte differentiation antigen CD36 antibody;MGC108510 antibody;MGC91634 antibody;PAS 4 protein antibody;PAS IV antibody;PAS-4 antibody;PASIV antibody;Platelet collagen receptor antibody;Platelet glycoprotein 4 antibody;Platelet glycoprotein IV antibody;scarb3 antibody;Scavenger receptor class B member 3 antibody;Thrombospondin receptor antibody
SEQUENCE SIMILARITIES
Belongs to the CD36 family.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated and O-glycosylated with a ratio of 2:1.; Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome. Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids.
SUBCELLULAR LOCATION
Cell membrane, Membrane raft, Golgi apparatus, Apical cell membrane.
FUNCTION
CD36 (cluster of differentiation 36), also known as platelet glycoprotein 4, fatty acid translocase (FAT), scavenger receptor class B member 3 (SCARB3), and glycoproteins 88 (GP88), IIIb (GPIIIB), or IV (GPIV) is a protein that in humans is encoded by the CD36 gene. The CD36 antigen is an integral membrane protein found on the surface of many cell types in vertebrate animals. It imports fatty acids inside cells and is a member of the class B scavenger receptor family of cell surface proteins. CD36 binds many ligands including collagen, thrombospondin, erythrocytes parasitized with Plasmodium falciparum, oxidized low density lipoprotein, native lipoproteins, oxidized phospholipids, and long-chain fatty acids. Work in genetically modified rodents suggest a role for CD36 in fatty acid metabolism, heart disease, taste, and dietary fat processing in the intestine. It may be involved in glucose intolerance, atherosclerosis, arterial hypertension, diabetes, cardiomyopathy, Alzheimer's disease and various cancers, mostly of epithelial origin (breast, prostate, ovary, and colon) and also for hepatic carcinoma and gliomas.
CITATIONS
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Liu Dan,et al
Chrysanthemum morifolium attenuates metabolic and alcohol-associated liver disease via gut microbiota and PPARα/γ activation
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WB
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Rat
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Citation
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Hypoxia Drives Material-Induced Heterotopic Bone Formation by Enhancing Osteoclastogenesis via M2/Lipid-Loaded Macrophage Axis
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inducer
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Mouse
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Citation
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Characterizing the obesogenic and fatty liver-inducing effects of Acetyl tributyl citrate (ATBC) plasticizer using both in vivo and in vitro models
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WB
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Mouse
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Citation
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Liang, M., Guo, X., Wu, H., & Zhong, Z.
Inducible co-stimulator inhibits lipid phagocytosis of human aortic smooth muscle cells by down-regulating CD36 expression
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IF
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Human
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Citation
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Biao, T., Cai-Feng, H., Xiao-Hong, L., Xiao-Li, C., Wen-Bei, L., Jun, W., Chao, C., & Tao, Y.
From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses
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WB
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Human
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Citation
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Activation of the PERK-CHOP signaling pathway during endoplasmic reticulum stress contributes to olanzapine-induced dyslipidemia
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WB
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Mouse
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Citation
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