PRODUCT CODE: ER1706-66

CD34 Rabbit Polyclonal Antibody (ER1706-66)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.
  • Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD34 antibody. Counter stained with hematoxylin.
Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD34 Rabbit Polyclonal Antibody (ER1706-66)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

TF-1, human brain tissue lysate, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

CD34

SYNONYMS

CD34 antibody; CD34 antigen antibody; CD34 molecule antibody; CD34_HUMAN antibody; Cluster designation 34 antibody; Hematopoietic progenitor cell antigen CD34 antibody; HPCA1 antibody; Mucosialin antibody; OTTHUMP00000034733 antibody; OTTHUMP00000034734 antibody

SEQUENCE SIMILARITIES

Belongs to the CD34 family.

TISSUE SPECIFICITY

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

DEVELOPMENTAL STAGE

On early hematopoietic progenitor cells.

POST-TRANSLATIONAL MODIFICATION

Highly glycosylated.; Phosphorylated on serine residues by PKC.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. However, little is known about its exact function. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.