PRODUCT CODE: ET1703-43

CD32 Recombinant Rabbit Monoclonal Antibody [JM10-70] (ET1703-43)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD32 on human lung cells lysates using anti-CD32 antibody at 1/500 dilution.
  • Western blot analysis of CD32 on human lung cells lysates using anti-CD32 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD32 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD32 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD32 antibody. Counter stained with hematoxylin.
Western blot analysis of CD32 on human lung cells lysates using anti-CD32 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD32 Recombinant Rabbit Monoclonal Antibody [JM10-70] (ET1703-43)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung, human tonsil tissue, human spleen tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-70

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD32

SYNONYMS

CD32 antibody; CD32A antibody; CD32B antibody; CD32C antibody; CDw32 antibody; Fc fragment of IgG low affinity IIa receptor antibody; Fc fragment of IgG low affinity IIa receptor for (CD32) antibody; Fc fragment of IgG low affinity IIb receptor antibody; Fc fragment of IgG low affinity IIb receptor for (CD32) antibody; Fc fragment of IgG low affinity IIc receptor antibody; Fc fragment of IgG low affinity IIc receptor for (CD32) antibody; Fc fragment of IgG, low affinity II, receptor for (CD32) antibody; Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody; Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody; Fc gamma receptor IIB antibody; Fc gamma receptor IIC antibody; Fc gamma RII a antibody; Fc gamma RII b antibody; Fc gamma RII c antibody; Fc gamma RIIb antibody; Fc gamma RIIc antibody; Fc receptor, IgG, low affinity IIb antibody; Fc-gamma RII-a antibody; Fc-gamma RII-b antibody; Fc-gamma RII-c antibody; Fc-gamma-RIIa antibody; FC-gamma-RIIB antibody; Fc-GAMMA-RIIC antibody; Fc[g]RII antibody; FCG2 antibody; FCG2A_HUMAN antibody; FcGR antibody; FCGR2 antibody; FCGR2A antibody; FCGR2A1 antibody; FCGR2B antibody; FCGR2C antibody; Fcgr3 antibody; Fcgr3a antibody; FcgRII antibody; Fcr-2 antibody; Fcr-3 antibody; FcRII a antibody; FCRII antibody; FcRII b antibody; FcRII c antibody; FcRII-a antibody; FcRIIC antibody; IGFR2 antibody; IgG Fc receptor II a antibody; IgG Fc receptor II b antibody; IgG Fc receptor II beta antibody; IgG Fc receptor II c antibody; IgG Fc receptor II-a antibody; IgG Fc receptor II-b antibody; IgG Fc receptor II-c antibody; Immunoglobulin G Fc receptor II antibody; Immunoglobulin G Fc receptor II c antibody; Low affinity immunoglobulin gamma Fc region receptor II antibody; Low affinity immunoglobulin gamma Fc region receptor II-a antibody; Low affinity immunoglobulin gamma Fc region receptor II-b antibody; Low affinity immunoglobulin gamma Fc region receptor II-c antibody; Ly-17 antibody; Ly-m20 antibody; LyM-1 antibody; Lymphocyte antigen 17 antibody

TISSUE SPECIFICITY

Found on monocytes, neutrophils and eosinophil platelets.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG (1,2). CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells (1-4). IgG can noncooperatively bind either one or two highly glycosylated CD32 molecules, and this binding delivers a negative signal for B cells (1,2,5). CD32 exists as several isoforms that are produced by alternative splicing of three distinct genes, A, B, and C (2,6). These isoforms are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC (1,2,6). All isoforms are present on monocytes, placental trophoblasts and endothelial cells (1,6). In addition, the FcgRIIB forms are present on B lymphocytes, and the FcgRIIA and FcgRIIC forms are found on neutrophils (1,6).