PRODUCT CODE: 0805-7

CD3 zeta Rabbit Polyclonal Antibody (0805-7)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of CD3 zeta on Jurkat cell lysate using anti-CD3 zeta antibody at 1/1,000 dilution.
  • Western blot analysis of CD3 zeta on Jurkat cell lysate using anti-CD3 zeta antibody at 1/1,000 dilution.
  • ICC staining CD3 zeta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD3 zeta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD3 zeta in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Jurkat cells with CD3 zeta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD3 zeta on Jurkat cell lysate using anti-CD3 zeta antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD3 zeta Rabbit Polyclonal Antibody (0805-7)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to a region of c-terminal residues of cd3 zeta.

Host

Rabbit

Positive Control

Jurkat, A549, Hela, HT-29.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

19kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD3 zeta

SYNONYMS

4930549J05Rik antibody; A430104F18Rik antibody; AW552088 antibody; CD247 antibody; CD247 antigen antibody; Cd247 molecule antibody; Cd3 antibody; CD3 antigen, zeta polypeptide, isoform CRA_b antibody; CD3 antigen, zeta subunit antibody; CD3-eta antibody; CD3H antibody; CD3Q antibody; Cd3z antibody; CD3Z antigen zeta polypeptide (TiT3 complex) antibody; CD3Z_HUMAN antibody; CD3zeta antibody; CD3zeta chain antibody; MGC140430 antibody; T cell receptor T3 zeta chain antibody; T cell surface glycoprotein CD3 zeta chain antibody; T-cell antigen receptor complex, zeta subunit of CD3 antibody; T-cell receptor T3 zeta chain antibody; T-cell surface glycoprotein CD3 zeta chain antibody; T3z antibody; TCR zeta chain antibody; TCRk antibody; TCRZ antibody; TCRzeta antibody

SEQUENCE SIMILARITIES

Belongs to the CD3Z/FCER1G family.

TISSUE SPECIFICITY

CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN).