CD3 zeta Recombinant Rabbit Monoclonal Antibody [JE09-37] (HA722692)
Catalog# HA722692
Rabbit Monoclonal to CD3 zeta
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD3 zeta on different lysates with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722692) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD3 zeta on different lysates with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722692) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.