CD3 epsilon Rabbit Polyclonal Antibody (ER80501)
Catalog# ER80501
Rabbit Polyclonal to CD3 epsilon
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WB
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
Lane 4: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 20 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CD3 epsilon Rabbit Polyclonal Antibody (ER80501)
Immunogen
Synthetic peptide within c-terminal human cd3 epsilon.
Host
Rabbit
Positive Control
HeLa cell lysate, Mouse spleen tissue lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD3 epsilon
SYNONYMS
CD3 epsilon antibody;CD3e antibody;CD3e antigen epsilon polypeptide (TiT3 complex) antibody;CD3E antigen epsilon polypeptide antibody;CD3E antigen, epsilon subunit antibody;CD3e molecule epsilon antibody;CD3e molecule, epsilon (CD3 TCR complex) antibody;CD3e molecule, epsilon (CD3-TCR complex) antibody;CD3E_HUMAN antibody;IMD18 antibody;T cell antigen receptor complex epsilon subunit of T3 antibody;T cell surface antigen T3/Leu 4 epsilon chain antibody;T cell surface glycoprotein CD3 epsilon chain antibody;T-cell surface antigen T3/Leu-4 epsilon chain antibody;T-cell surface glycoprotein CD3 epsilon chain antibody;T3E antibody;TCRE antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) These chains associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes.
CITATIONS
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Zhang, M., Chong, K. K., Chen, Z. Y., Guo, H., Liu, Y. F., Kang, Y. Y., Li, Y. J., Shi, T. T., Lai, K. K., He, M. Q., Ye, K., Kahaly, G. J., Shi, B. Y., & Wang, Y.
Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes
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IHC-P
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Mouse
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Citation
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