Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD27 Recombinant Rabbit Monoclonal Antibody [JB40-98] (ET7107-73)
Immunogen
Intracellular domain corresponding to human cd27.
Host
Rabbit
Positive Control
Raji cell lysates, K562, human tonsil tissue, rat spleen tissue, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB40-98
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
Predicted band size: 29 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD27
SYNONYMS
CD 27 antibody; CD27 antibody; CD27 antigen antibody; CD27 molecule antibody; CD27_HUMAN antibody; CD27L receptor antibody; LPFS2 antibody; MGC20393 antibody; OTTHUMP00000238557 antibody; S152 antibody; T cell activation antigen CD27 antibody; T cell antivation antigen S152 antibody; T-cell activation antigen CD27 antibody; T14 antibody; TNFRSF 7 antibody; TNFRSF7 antibody; TNFSF7 antibody; Tp 55 antibody; Tp55 antibody; Tumor necrosis factor receptor superfamily member 7 antibody
TISSUE SPECIFICITY
Found in most T-lymphocytes.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated.; O-glycosylated with core 1 or possibly core 8 glycans.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cysteine-rich extracellular domains. Members of this family include FAS, OX40, CD27 and CD30. Ligands for these receptors are often type II transmembrane glycoproteins, as is the case for CD27 and CD30. CD27 is a homodimeric lymphocyte-specific surface antigen present on T and B lymphocytes. Activation of the CD3 complex via the T cell receptor for antigen leads to an increase in CD27 expression. Together, CD27 and its ligand, CD27L, generate co-stimulatory signals required for complete T cell activation. CD30 is a surface marker for neoplastic cells of the Hodgkin's lymphoma and related hematologic malignancies. CD30L has been shown to enhance the proliferation of the Hodgkin's cell line HDLM-2, but exerts antiproliferative effects on large cell anaplastic lymphoma cell lines.