PRODUCT CODE: ET1703-93

CD26 Recombinant Rabbit Monoclonal Antibody [JM11-42] (ET1703-93)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse lung tissue lysate<br />
Lane 2: mouse heart tissue lysate<br />
Lane 3: human kidney tissue lysate<br />
Lane 4: Raji cell lysate<br />
Lane 5: PC-3 cell lysate
  • Western blot analysis of CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse lung tissue lysate<br />
Lane 2: mouse heart tissue lysate<br />
Lane 3: human kidney tissue lysate<br />
Lane 4: Raji cell lysate<br />
Lane 5: PC-3 cell lysate
  • ICC staining of CD26 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD26 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CD26 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-93, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse lung tissue lysate
Lane 2: mouse heart tissue lysate
Lane 3: human kidney tissue lysate
Lane 4: Raji cell lysate
Lane 5: PC-3 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD26 Recombinant Rabbit Monoclonal Antibody [JM11-42] (ET1703-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse lung tissue lysate, mouse heart tissue lysate, human kidney tissue lysate, Raji cell lysate, PC-3 cell lysate, Hela, PC-12, human liver tissue, rat epididymis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-42

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

110 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD26

SYNONYMS

CD26 antigen antibody; ADA-binding protein antibody; ADABP antibody; ADCP 2 antibody; ADCP-2 antibody; ADCP2 antibody; Adenosine deaminase complexing protein 2 antibody; CD 26 antibody; CD26 antibody; CD26 antigen 3 antibody; Dipeptidyl peptidase 4 antibody; Dipeptidyl peptidase 4 soluble form antibody; Dipeptidyl peptidase IV antibody; Dipeptidyl peptidase IV membrane form antibody; Dipeptidyl peptidase IV soluble form antibody; Dipeptidyl peptidase, intestinal antibody; Dipeptidylpeptidase 4 antibody; Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody; Dipeptidylpeptidase IV antibody; DPP 4 antibody; DPP IV antibody; DPP IV estoenzyme antibody; DPP4 antibody; DPP4_HUMAN antibody; DPPIV antibody; Intestinal dipeptidyl peptidase antibody; T cell activation antigen CD26 antibody; T-cell activation antigen CD26 antibody; TP 103 antibody; TP103 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S9B family. DPPIV subfamily.

TISSUE SPECIFICITY

Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

POST-TRANSLATIONAL MODIFICATION

The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.; N- and O-Glycosylated.; Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.

SUBCELLULAR LOCATION

Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft.

FUNCTION

CD26 (dipeptidylpeptidase 4, adenosine deaminase complexing protein 2, ADABP, ADCP2, DPPIV, TP103) is a membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. CD26 has an essential role in immune regulation as a T cell activation molecule and a regulator of chemokine function. CD26 associates with CXCR4 and gp120 and may influence the pathophysiology of HIV infection. Adenosine deaminase (ADA) colocalizing with adenosine receptors on dendritic cells are able to interact with CD26 expressed on lymphocytes. This costimulatory signal in the immunological synapse leads to an increase in the production of the T helper 1 and proinflammatory cytokines IFN-gamma, TNF-alpha, and IL-6. CD26 plays a role in the pathogenesis and behavior of human cancers, including solid tumors and hematological malignancies. CD26-caveolin-1 interaction plays a role in the up-regulation of CD86 on TT-loaded monocytes and subsequent engagement with CD28 on T cells, leading to antigen-specific T cell activation.