PRODUCT CODE: 0804-4

CD24 Rabbit Polyclonal Antibody (0804-4)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of CD24 on human spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD24 on human spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of CD24 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (0804-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD24 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (0804-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of CD24 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (0804-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD24 on human spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD24 Rabbit Polyclonal Antibody (0804-4)

Immunogen

Synthetic peptide within human cd24 aa 31-80 / 80.

Host

Rabbit

Positive Control

Human spleen,Hela

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

39kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:100-500

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

CD24

SYNONYMS

CD 24 antibody; CD24 antibody; CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody; CD24 antigen antibody; CD24 molecule antibody; CD24_HUMAN antibody; CD24A antibody; FLJ22950 antibody; FLJ43543 antibody; GPI linked surface mucin antibody; Heat stable antigen antibody; HSA antibody; MGC75043 antibody; Nectadrin antibody; Signal transducer CD24 antibody; Small cell lung carcinoma cluster 4 antigen antibody

SEQUENCE SIMILARITIES

Belongs to the CD24 family.

TISSUE SPECIFICITY

B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

POST-TRANSLATIONAL MODIFICATION

Extensively O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

The human CD24 antigen is a small heavily glycosylated, glycosylphosphatidylinositol (GPI) linked cell surface protein. It consists of a small protein core comprising 27 amino acids, which is extensively glycosylated and is bound to the membrane via a phosphatidylinositol anchor. The human CD24 antigen is physiologically expressed in developing or regenerating tissue, as well as in granulocytes, keratinocytes and renal tubular epithelium. It modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor

CITATIONS

  • Xiang, Xiaocong et al.

    Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/β-catenin pathway. | Oncology Reports [2019]