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PRODUCT CODE: 0804-3

CD24 Rabbit Polyclonal Antibody (0804-3)

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

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Western blot analysis of CD24 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD24 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD24 on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-3, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD24 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0804-3, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CD24 was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (0804-3, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Western blot analysis of CD24 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD24 Rabbit Polyclonal Antibody (0804-3)

Immunogen

Synthetic peptide within mouse cd24 aa 27-76 / 76.

Host

Rabbit

Positive Control

Mouse brain tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

37kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

P24807

PROTEIN NAME

CD24

SYNONYMS

CD 24 antibody; CD24 antibody; CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody; CD24 antigen antibody; CD24 molecule antibody; CD24_HUMAN antibody; CD24A antibody; FLJ22950 antibody; FLJ43543 antibody; GPI linked surface mucin antibody; Heat stable antigen antibody; HSA antibody; MGC75043 antibody; Nectadrin antibody; Signal transducer CD24 antibody; Small cell lung carcinoma cluster 4 antigen antibody

SEQUENCE SIMILARITIES

Belongs to the CD24 family.

TISSUE SPECIFICITY

In lymphoid, myeloid, and erythroid cells.

POST-TRANSLATIONAL MODIFICATION

The identity of the N- and O-linked polysaccharides are not reported in PubMed:1530634. The O-linked polysaccharides on Ser-30, Ser-41, Ser-43, and Thr-51 are probably the mucin type linked to GalNAc.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

The CD24 antigen is a small heavily glycosylated, glycosylphosphatidylinositol (GPI) linked cell surface protein. It consists of a small protein core comprising 27 amino acids, which is extensively glycosylated and is bound to the membrane via a phosphatidylinositol anchor. The CD24 antigen is physiologically expressed in developing or regenerating tissue, as well as in granulocytes, keratinocytes and renal tubular epithelium. It modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor.