CD200 Recombinant Rabbit Monoclonal Antibody [PSH01-49] (HA721692)
Catalog# HA721692
Rabbit Monoclonal to CD200
Tested for
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WB
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IHC-P
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FC
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IF-Tissue
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD200 on different lysates with Rabbit anti-CD200 antibody (HA721692) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: NCI-H226 cell lysate (20 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: Human lung tissue lysate (20 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 40-50 kDa
Exposure time: 2 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721692) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: NCI-H226 cell lysate (20 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: Human lung tissue lysate (20 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 40-50 kDa
Exposure time: 2 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721692) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.