PRODUCT CODE: ET1605-33

CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01] (ET1605-33)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Monkey

Western blot analysis of CD20 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD20 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD20 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Monkey

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01] (ET1605-33)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Raji cell lysates, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY12-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD20

SYNONYMS

APY antibody; ATOPY antibody; B lymphocyte antigen CD20 antibody; B Lymphocyte Cell Surface Antigen B1 antibody; B-lymphocyte antigen CD20 antibody; B-lymphocyte cell-surface antigen B1 antibody; B-lymphocyte surface antigen B1 antibody; B1 antibody; Bp 35 antibody; Bp35 antibody; CD 20 antibody; CD20 antibody; CD20 antigen antibody; CD20 receptor antibody; CD20_HUMAN antibody; CVID5 antibody; Fc epsilon receptor I beta chain antibody; Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody; FCER1B antibody; High affinity immunoglobulin epsilon receptor subunit beta antibody; IgE Fc receptor subunit beta antibody; IGEL antibody; IGER antibody; IGHER antibody; LEU 16 antibody; LEU16 antibody; leukocyte surface antigen Leu 16 antibody; Leukocyte surface antigen Leu-16 antibody; Ly44 antibody; Membrane spanning 4 domains A1 antibody; Membrane spanning 4 domains subfamily A member 2 antibody; Membrane-spanning 4-domains subfamily A member 1 antibody; MS4A1 antibody; MS4A2 antibody; S7 antibody

SEQUENCE SIMILARITIES

Belongs to the MS4A family.

TISSUE SPECIFICITY

Expressed on B-cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, leading to additional isoforms. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cation channel, and it is known to accelerate the G0 to G1 progression induced by IGF-1. CD20 is activated by the IGF-1 receptor via the alpha subunits of the heterotrimeric G proteins. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.