PRODUCT CODE: R1107-7

CD19 Rabbit Polyclonal Antibody (R1107-7)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.
  • Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.
Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD19 Rabbit Polyclonal Antibody (R1107-7)

Immunogen

Peptide

Host

Rabbit

Positive Control

NCCIT, F9

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

80-95kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

CD19

SYNONYMS

antibody; deficiency due to defect in CD19, included antibody; AW495831 antibody; B lymphocyte antigen CD19 antibody; B lymphocyte surface antigen B4 antibody; B-lymphocyte antigen CD19 antibody; B-lymphocyte surface antigen B4 antibody; B4 antibody; CD19 antibody; CD19 antigen antibody; CD19 molecule antibody; Cd19 protein antibody; CD19_HUMAN antibody; CVID3 antibody; Differentiation antigen CD19 antibody; Leu 12 antibody; Leu-12 antibody; Leu12 antibody; MGC109570 antibody; MGC12802 antibody; T-cell surface antigen Leu-12 antibody

TISSUE SPECIFICITY

Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

B-lymphocyte antigen CD19 also known as CD19 (Cluster of Differentiation 19), is a 95 kDa type-I transmembrane glycoprotein which is restricted to B cell antigen. On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. CD19 is also found on the follicular dendritic cells and the early cells of myelomonocytic lineage but not on normal T cells, NK cells, monocytes, granulocytes, erythrocytes and platelets.