CD19 Mouse Monoclonal Antibody [A3-A8] (EM40308)
Catalog# EM40308
Mouse Monoclonal to CD19
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WB
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IHC-P
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FC
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Human
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Mouse
Positive control:
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Specifications
Product Type
Mouse monoclonal primary
Product Name
CD19 Mouse Monoclonal Antibody [A3-A8] (EM40308)
Immunogen
Synthetic peptide within human cd19 aa 110-190.
Host
Mouse
Positive Control
Raji cell lysate, Daudi cell lysate, mouse lymphoid lysates, human spleen tissue, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3-A8
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:200
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FC
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1:100-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 61 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
CD19
SYNONYMS
antibody;deficiency due to defect in CD19, included antibody;AW495831 antibody;B lymphocyte antigen CD19 antibody;B lymphocyte surface antigen B4 antibody;B-lymphocyte antigen CD19 antibody;B-lymphocyte surface antigen B4 antibody;B4 antibody;CD19 antibody;CD19 antigen antibody;CD19 molecule antibody;Cd19 protein antibody;CD19_HUMAN antibody;CVID3 antibody;Differentiation antigen CD19 antibody;Leu 12 antibody;Leu-12 antibody;Leu12 antibody;MGC109570 antibody;MGC12802 antibody;T-cell surface antigen Leu-12 antibody
TISSUE SPECIFICITY
Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).
SUBCELLULAR LOCATION
Membrane.
FUNCTION
B-lymphocyte antigen CD19 also known as CD19 (Cluster of Differentiation 19), is a 95 kDa type-I transmembrane glycoprotein which is restricted to B cell antigen. On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. CD19 is also found on the follicular dendritic cells and the early cells of myelomonocytic lineage but not on normal T cells, NK cells, monocytes, granulocytes, erythrocytes and platelets.
CITATIONS
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Xianjun Zhu,Yiguo Hu
Tmem30a plays critical roles in ensuring the survival of hematopoietic cells and leukemia cells in mice
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IHC
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Mouse
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Citation
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