CD166 Mouse Monoclonal Antibody [A6A9] (HA600082)
Catalog# HA600082
Mouse Monoclonal to CD166
Tested for
-
IF-Cell
-
IHC-P
-
WB
Reacts with
-
Human
Knock-Out or Knock-Down validated
Western blot analysis of CD166 on different lysates with Mouse anti-CD166 antibody (HA600082) at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate
Lane 2: A549 cell lysate
Lane 3: LNCaP cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 100-105 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-2 OS cell lysate
Lane 2: A549 cell lysate
Lane 3: LNCaP cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 100-105 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.