CD166 Mouse Monoclonal Antibody [5-5] (EM1902-36)
Catalog# EM1902-36
Mouse Monoclonal to CD166
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
-
Human
Knock-Out or Knock-Down validated
Western blot analysis of CD166 on different lysates with Mouse anti-CD166 antibody (EM1902-36) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD CD166 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-36) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD CD166 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-36) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.