Positive control:
Lane 1: PC-3M cell lysate
Lane 2: A549 cell lysate
Lane 3: SHSY5Y cell lysate
Applications
-
WB
-
IHC-P
-
ICC
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
CD166 Mouse Monoclonal Antibody [5-5] (EM1902-36)
Immunogen
Recombinant protein within human cd166 aa 1-300.
Host
Mouse
Positive Control
PC-3M cell lysate, A549 cell lysate, SHSY5Y cell lysate, A431cells, Huvec celles, human liver tissue, THP-1 cells.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
5-5
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
Predicted band size: 65 kDa.
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:50-1:100
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD166
SYNONYMS
Activated leukocyte cell adhesion molecule antibody; ALCAM antibody; ALCAM protein antibody; CD 166 antibody; CD166 antibody; CD166 antigen antibody; CD166_HUMAN antibody; FLJ3851 antibody; FLJ38514 antibody; MEMD antibody; MGC71733 antibody
SUBCELLULAR LOCATION
Cell membrane, Cell projection, Membrane, Secreted
FUNCTION
Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts . Promotes T-cell activation and proliferation via its interactions with CD6 . Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 . Mediates homotypic interactions with cells that express ALCAM . Required for normal hematopoietic stem cell engraftment in the bone marrow . Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction . Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions . Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).