CD163 Rabbit Polyclonal Antibody (ER1804-03)
Catalog# ER1804-03
Rabbit Polyclonal to CD163
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: Human lung tissue lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 125 kDa
Observed band size: 150-170 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1804-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CD163 Rabbit Polyclonal Antibody (ER1804-03)
Immunogen
Recombinant protein within human aa 22-161 / 1,156.
Host
Rabbit
Positive Control
Human lung tissue lysate, human liver tissue lysate, LOVO, SH-SY5Y, Siha, A431, mouse liver tissue, rat lung tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB:1:500-1:1,000
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IF-Cell:1:50-1:200
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IHC-P:1:50-1:600
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 125 kDa, observed band size: 150 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD163
SYNONYMS
C163A_HUMAN antibody;CD 163 antibody;CD163 antibody;CD163 antigen antibody;CD163 molecule antibody;Hemoglobin scavenger receptor antibody;M130 antibody;M130 antigen precursor antibody;Macrophage associated antigen antibody;MM130 antibody;OTTHUMP00000238617 antibody;OTTHUMP00000238618 antibody;OTTHUMP00000238619 antibody;OTTHUMP00000238620 antibody;SCARI1 antibody;Scavenger receptor cysteine rich type 1 protein M130 antibody;sCD163 antibody;Soluble CD163 antibody
TISSUE SPECIFICITY
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
POST-TRANSLATIONAL MODIFICATION
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.; Phosphorylated.
SUBCELLULAR LOCATION
Secreted, Cell membrane.
FUNCTION
CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31.
CITATIONS
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Wang, X., Yu, Y., Yang, C., Shang, L., Zhao, Y., & Shen, X.
Dynamically Responsive Scaffolds from Microfluidic 3D Printing for Skin Flap Regeneration
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IHC-P
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Mouse
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Citation
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Bai, R., Wu, D., Shi, Z., Hu, W., Li, J., Chen, Y., Ge, W., Yuan, Y., & Zheng, S.
Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer. Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 33(1), 93–102.
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IHC
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Human
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Citation
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Feng Zhuan,et al
Inhibition of Ferroptosis Rescues M2 Macrophages and Alleviates Arthritis by Suppressing the HMGB1/TLR4/STAT3 Axis in M1 Macrophages
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IHC
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Mouse
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Citation
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Li, H., Yuan, Y., Chen, H., Dai, H., & Li, J.
Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway
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ICC
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Rat
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Citation
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