CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79] (ET7109-97)
Catalog# ET7109-97
Rabbit Monoclonal to CD16
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WB
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Human
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Mouse
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Rat
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Lane 5: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 40 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79] (ET7109-97)
Immunogen
Recombinant protein within human cd16 aa 151-254 / 254.
Host
Rabbit
Positive Control
THP-1 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, rat spleen tissue lysate, rat thymus tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE49-79
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD16
SYNONYMS
CD 16 antibody;CD 16a antibody;CD16 antibody;CD16a antibody;CD16a antigen antibody;CD16B antibody;CD16b antigen antibody;Fc fragment of IgG antibody;Fc fragment of IgG low affinity IIIa receptor (CD16) antibody;Fc fragment of IgG low affinity IIIa receptor antibody;Fc fragment of IgG receptor IIIa antibody;Fc fragment of IgG, low affinity III, receptor (CD16) antibody;Fc fragment of IgG, low affinity III, receptor for (CD16) antibody;Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody;Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody;Fc fragment of IgG, low affinity IIIa, receptor for antibody;Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody;Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody;Fc gamma R3 antibody;Fc gamma receptor III 2 (CD 16) antibody;Fc gamma receptor III A antibody;Fc gamma receptor IIIA antibody;Fc gamma receptor IIIb (CD 16) antibody;Fc gamma RIII alpha antibody;Fc gamma RIII antibody;Fc gamma RIII beta antibody;Fc gamma RIIIa antibody;Fc gamma RIIIb antibody;Fc of IgG antibody;Fc-gamma receptor III2 (CD 16) antibody;Fc-gamma receptor III2 (CD16) antibody;Fc-gamma receptor IIIb (CD16) antibody;Fc-gamma RIII antibody;Fc-gamma RIII-alpha antibody;Fc-gamma RIIIa antibody;FCG 3 antibody;FCG3 antibody;FCG3A_HUMAN antibody;FCgammaRIIIA antibody;FCGR 3 antibody;FCGR 3A antibody;FCGR3 antibody;FCGR3A antibody;FCGR3A protein antibody;FCGRIII antibody;FCGRIII-2 antibody;FcR 10 antibody;FcR-10 antibody;FcR10 antibody;FcRIII antibody;FcRIIIa antibody;IGFR 3 antibody;IGFR3 antibody;IgG Fc receptor III 1 antibody;IgG Fc receptor III 2 antibody;IgG Fc receptor III-2 antibody;IMD20 antibody;immunoglobulin G Fc receptor III antibody;immunoglobulin G Fc receptor III-2 antibody;Low affinity IIIa receptor antibody;Low affinity immunoglobulin gamma Fc region receptor III A antibody;Low affinity immunoglobulin gamma Fc region receptor III-A antibody;Low affinity immunoglobulin gamma Fc region receptor IIIB antibody;neutrophil-specific antigen NA antibody
TISSUE SPECIFICITY
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
POST-TRANSLATIONAL MODIFICATION
Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.; The soluble form is produced by a proteolytic cleavage.
SUBCELLULAR LOCATION
Cell membrane, Membrane, Secreted.
FUNCTION
CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.
CITATIONS
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Zha, Z., Hong, Y., Tang, Z., Du, Q., Wang, Y., Yang, S., Wu, Y., Tan, H., Jiang, F., & Zhong, W.
FCGR3A: A new biomarker with potential prognostic value for prostate cancer
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IHC-P
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Human
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Citation
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